Evidence map›Paper›PMID 42844280›Full record

ArticleNature communications2026

SPE-55 and VHA-18 are essential for FB-MO complex assembly and transport during spermatogenesis in C. elegans.

Xiaojing Ma, Xinyan Chen, Lianwan Chen, Peng Wang, Xinyue Lu, Xiaolong Zhang, Ying Chen, Ning Wang, Qiushi Wang, Zheng Cao and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xiaojing Ma *MOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
Xinyan Chen *Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.ORCID 0009-0008-4129-1349
Lianwan Chen *Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Peng Wang *Anhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin, School of Biology and Food Engineering, Fuyang Normal University, Fuyang, China. wangpeng@fynu.edu.cn.ORCID 0009-0005-5686-0995
Xinyue LuMOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
Xiaolong ZhangMOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
Ying ChenMOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
Ning WangMOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
Qiushi WangKey Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Zheng CaoKey Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Yong LiuAnhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin, School of Biology and Food Engineering, Fuyang Normal University, Fuyang, China. liuyong@fynu.edu.cn.ORCID 0000-0002-0583-072X
Yanmei ZhaoKey Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. ymzhao@genetics.ac.cn.
Long MiaoMOE Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Sciences, Beijing Normal University, Beijing, China. lmiao@bnu.edu.cn.ORCID 0000-0002-0562-6681

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32070694National Natural Science Foundation of China (National Science Foundation of China) 32400698
6 · The paper itself

Abstract

The spatiotemporal control of asymmetric positioning of cellular cargoes, such as macromolecules and organelles, during spermatogenesis is essential for producing functional sperm in most animal species. However, the regulatory mechanisms that regulate cargo sorting and transporting remain largely unknown. Here, we demonstrate that the casein-like kinase SPE-55 localized on the surface of a membranous organelle (MO) is required for the SPE-18 localization/puncta formation and facilitates the assembly of major sperm protein (MSP) condensate, named the fibrous body (FB), in spermatocytes during spermatogenesis in Caenorhabditis elegans. Furthermore, we discover that the H subunit of v-ATPase, which is localized on MOs, played a crucial role in maintaining MO morphology and function, promoting FB-MO connections, and thus ensuring efficient MSP sorting during spermatogenesis. Therefore, the interplay between the membraneless condensate FBs mediated by SPE-55 and the membrane-bound MOs regulated by VHA-18 provides a coordinated mechanism for cargo sorting and directional transport during spermatogenesis in C. elegans.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsOrganellesSpermatogenesisAnimalsHelminth ProteinsMaleProtein TransportSpermatocytesSperm ProteinsVacuolar Proton-Translocating ATPasesCaenorhabditis elegans ProteinsHelminth Proteinsmajor sperm protein, nematodeSperm ProteinsVacuolar Proton-Translocating ATPases

Identifiers

PMID42844280
PMCPMC13645734

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.