ArticleAdvanced biotechnology2026
A single-cell profile of the porcine immune repertoire: concurrent profiling of T cell receptor and B cell receptor V(D)J sequences in health.
Article in Advanced biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Adaptive immune diversity is shaped by the complex organization of T cell receptor (TCR) and B cell receptor (BCR) repertoires across tissues. However, a systematic cross-tissue immune repertoire profiling in healthy pigs is still lacking. In this study, we used 3 14-day-old Duroc × Landrace × Yorkshire (DLY) crossbred piglets, all of which were male. After sample purification, a total of 71,731 cells were retained. We established single-cell profiling integrating paired BCR and TCR repertoires across 11 immune-associated tissues using single-cell RNA sequencing combined with V(D)J sequencing based on DLY crossbred piglets. Diverse populations of immune cells were identified, with significant tissue-specific heterogeneity being revealed in lymphatic, mucosal, and circulatory organs. Immune repertoire analysis demonstrated biased V(D)J gene usage, conserved complementarity-determining region 3 (CDR3) length distributions, preferential receptor pairing patterns, and extensive clonotype diversity. Cross-tissue analyses further revealed coordinated clonotype sharing and distinct clonal expansion patterns among immune cell subsets. In addition, using the Pig-specific V(D)J primers we designed, the sequencing results have achieved a modest improvement in the detection of immunoglobulin alleles compared to previous studies. It is expected to provide a more reliable technical solution for related research. Collectively, this work provides a comprehensive reference profiling of the healthy DLY crossbred piglet adaptive immune system and offers valuable resources for future studies of porcine immunology, infectious diseases, and vaccine responses.
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