ArticlePflugers Archiv : European journal of physiology1995
A fluorimetric and amperometric study of calcium and secretion in isolated mouse pancreatic beta-cells.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 79 citations in OpenAlex.
- Insulin Release Mechanism Modulated by Toxins Isolated from Animal Venoms: From Basic Research to Drug Development Prospects.Molecules (Basel, Switzerland) · 2019Review
- Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men.Physiological reviews · 2018Review
- Article
- Human Beta Cells Produce and Release Serotonin to Inhibit Glucagon Secretion from Alpha Cells.Cell reports · 2016Article
- Dopamine-mediated autocrine inhibitory circuit regulating human insulin secretion in vitro.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Modes of exocytosis and electrogenesis underlying canine biphasic insulin secretion.Frontiers in bioscience (Elite edition) · 2012Review
- Article
- A fast solution switching system with temperature control for single cell measurements.Journal of neuroscience methods · 2011Article
- Exocytosis from pancreatic β-cells: mathematical modelling of the exit of low-molecular-weight granule content.Interface focus · 2011Article
- Quantal ATP release in rat beta-cells by exocytosis of insulin-containing LDCVs.Pflugers Archiv : European journal of physiology · 2009Article
- cAMP increases Ca2+-dependent exocytosis through both PKA and Epac2 in mouse melanotrophs from pituitary tissue slices.The Journal of physiology · 2005Article
- Maintenance of stimulus-secretion coupling and single beta-cell function in cryopreserved-thawed human islets of Langerhans.Pflugers Archiv : European journal of physiology · 2005Article
- alpha-Latrotoxin increases spontaneous and depolarization-evoked exocytosis from pancreatic islet beta-cells.The Journal of physiology · 2005Article
- A highly Ca2+-sensitive pool of granules is regulated by glucose and protein kinases in insulin-secreting INS-1 cells.The Journal of general physiology · 2004Article
- Insulin granule dynamics in pancreatic beta cells.Diabetologia · 2003Review
- Amplification of exocytosis by Ca2+-induced Ca2+ release in INS-1 pancreatic beta cells.The Journal of physiology · 2003Article
- Microdomains of high calcium are not required for exocytosis in RBL-2H3 mucosal mast cells.The Journal of cell biology · 2001Article
- Regulation of exocytosis by protein kinases and Ca(2+) in pancreatic duct epithelial cells.The Journal of general physiology · 2000Article
- Quantal analysis of 5-hydroxytryptamine release from mouse pancreatic beta-cells.The Journal of physiology · 1999Article
- Influence of cell number on the characteristics and synchrony of Ca2+ oscillations in clusters of mouse pancreatic islet cells.The Journal of physiology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We have examined the temporal relationship between intracellular Ca2+ concentration ([Ca2+]i) and secretion in single intact pancreatic beta-cells. Secretion was detected as the release of 5-hydroxytryptamine from pre-loaded beta-cells, using amperometry, and changes in [Ca2+]i were monitored by microfluorimetry. Stimulation of beta-cells by elevation of the extracellular K+ concentration ([K+]o), acetylcholine or glucose increased [Ca2+]i and, after a delay of 2-7 s, evoked amperometric currents. In the presence of glucose, we observed oscillations in [Ca2+]i which were associated with oscillations in the amplitude and frequency of amperometric currents: however, the temporal correlation was not exact, suggesting that there is a significant latency between the increase in average [Ca2+]i and exocytosis. Both the amplitude and frequency of the amperometric currents elicited by 50 mM KCl declined with successive stimulation, but were restored by agents which elevate intracellular adenosine 3':5':cyclic monophosphate (cAMP). This suggests that beta-cells may possess a readily releasable pool of granules which is replenished by cAMP. The variable amplitude of the amperometric currents is discussed in terms of a model in which several secretory granules fuse simultaneously with the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.