ArticleMolecular and cellular biology1994
Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 111 citations in OpenAlex.
- The Configuration of GRB2 in Protein Interaction and Signal Transduction.Biomolecules · 2024Review
- Antioxidant, Anti-Inflammatory and Antiproliferative Effects ofPlants (Basel, Switzerland) · 2023Article
- Cell cycle control by the insulin-like growth factor signal: at the crossroad between cell growth and mitotic regulation.Cell cycle (Georgetown, Tex.) · 2023Review
- KRAS G12 isoforms exert influence over up-front treatments: A retrospective, multicenter, Italian analysis of the impact of first-line immune checkpoint inhibitors in an NSCLC real-life population.Frontiers in oncology · 2022Article
- Article
- Ras signals principally via Erk in G1 but cooperates with PI3K/Akt for Cyclin D induction and S-phase entry.Cell cycle (Georgetown, Tex.) · 2019Article
- RAS: Striking at the Core of the Oncogenic Circuitry.Frontiers in oncology · 2019Review
- Feedback activation of neurofibromin terminates growth factor-induced Ras activation.Cell communication and signaling : CCS · 2016Article
- 5,8-Dimethoxy-2-Nonylamino-Naphthalene-1,4-Dione Inhibits Vascular Smooth Muscle Cell Proliferation by Blocking Autophosphorylation of PDGF-Receptor β.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Resveratrol inhibits PDGF receptor mitogenic signaling in mesangial cells: role of PTP1B.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Prediction of phosphotyrosine signaling networks using a scoring matrix-assisted ligand identification approach.Nucleic acids research · 2008Article
- NF-kappaB controls growth of glioblastomas/astrocytomas.Molecular and cellular biochemistry · 2008Article
- Additive effects of PDGF receptor beta signaling pathways in vascular smooth muscle cell development.PLoS biology · 2003Article
- Protein kinase PKR is required for platelet-derived growth factor signaling of c-fos gene expression via Erks and Stat3.The EMBO journal · 2001Article
- A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42.The Journal of cell biology · 2001Article
- Cholera toxin treatment of vascular smooth muscle cells decreases smooth muscle alpha-actin content and abolishes the platelet-derived growth factor-BB-stimulated DNA synthesis.British journal of pharmacology · 2000Article
- P2Y(2) receptor-mediated proliferation of C(6) glioma cells via activation of Ras/Raf/MEK/MAPK pathway.British journal of pharmacology · 2000Article
- Signaling in leukemia: which messenger to kill?The Journal of clinical investigation · 2000Article
- Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor.The Biochemical journal · 1999Article
- Association and direct activation of signal transducer and activator of transcription1alpha by platelet-derived growth factor receptor.The Journal of clinical investigation · 1998Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ligand stimulation of the platelet-derived growth factor (PDGF) beta-receptor leads to activation of its intrinsic tyrosine kinase and autophosphorylation of the intracellular part of the receptor. The autophosphorylated tyrosine residues mediate interactions with downstream signal transduction molecules and thereby initiate different signalling pathways. A pathway leading to activation of the GTP-binding protein Ras involves the adaptor molecule GRB2. Here we show that Tyr-716, a novel autophosphorylation site in the PDGF beta-receptor kinase insert, mediates direct binding of GRB2 in vitro and in vivo. In a panel of mutant PDGF beta-receptors, in which Tyr-716 and the previously known autophosphorylation sites were individually mutated, only PDGFR beta Y716F failed to bind GRB2. Furthermore, a synthetic phosphorylated peptide containing Tyr-716 bound GRB2, and this peptide specifically interrupted the interaction between GRB2 and the wild-type receptor. In addition, the Y716(P) peptide significantly decreased the amount of GTP bound to Ras in response to PDGF in permeabilized fibroblasts as well as in porcine aortic endothelial cells expressing transfected PDGF beta-receptors. The mutant PDGFR beta Y716F still mediated activation of mitogen-activated protein kinases and an increased DNA synthesis in response to PDGF, indicating that multiple signal transduction pathways transduce mitogenic signals from the activated PDGF beta-receptor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.