ArticleThe Biochemical journal1994
Lysophosphatidic acid inhibits gap-junctional communication and stimulates phosphorylation of connexin-43 in WB cells: possible involvement of the mitogen-activated protein kinase cascade.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 72 citations in OpenAlex.
- Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing.International journal of molecular sciences · 2021Review
- Targeting Lysophosphatidic Acid in Cancer: The Issues in Moving from Bench to Bedside.Cancers · 2019Review
- Regulation of oocyte meiotic maturation by somatic cells.Reproductive medicine and biology · 2012Article
- Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate.The Journal of cell biology · 2007Article
- Regulation of gap junctions by tyrosine protein kinases.Biochimica et biophysica acta · 2004Review
- Effect of angiotensin II and ethanol on the expression of connexin 43 in WB rat liver epithelial cells.The Biochemical journal · 2001Article
- A novel role for FGF and extracellular signal-regulated kinase in gap junction-mediated intercellular communication in the lens.The Journal of cell biology · 2001Article
- Direct evidence that ERK regulates the production/secretion of interleukin-2 in PHA/PMA-stimulated T lymphocytes.Immunology · 1999Article
- Dissection of the molecular basis of pp60(v-src) induced gating of connexin 43 gap junction channels.The Journal of cell biology · 1999Article
- Role of the extracellular signal-regulated protein kinase cascade in human neutrophil killing of Staphylococcus aureus and Candida albicans and in migration.Infection and immunity · 1999Article
- Lysophosphatidic acid stimulates neurotransmitter-like conductance changes that precede GABA and L-glutamate in early, presumptive cortical neuroblasts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Acute loss of cell-cell communication caused by G protein-coupled receptors: a critical role for c-Src.The Journal of cell biology · 1998Article
- Ventricular zone gene-1 (vzg-1) encodes a lysophosphatidic acid receptor expressed in neurogenic regions of the developing cerebral cortex.The Journal of cell biology · 1996Article
- A novel mechanism for persistence of human cytomegalovirus in macrophages.Journal of virology · 1996Article
- Cyclic AMP induces rapid increases in gap junction permeability and changes in the cellular distribution of connexin43.The Journal of membrane biology · 1995Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysophosphatidic acid (LPA) was shown to be a powerful inhibitor of gap-junctional communication between cultured rat liver WB cells, as determined by the transfer of Lucifer Yellow, with 50% inhibition obtained at about 0.3 microM LPA. Inhibition of communication was rapid (5 min) and was maintained for at least 80 min. After incubation for 3 h with LPA, communication competence was partially restored and dye transfer was refractory to further addition of LPA. Communication in LPA-refractory cells retained sensitivity to inhibition by phorbol ester and by epidermal growth factor (EGF). LPA-induced inhibition was associated with phosphorylation of connexin-43 protein, as detected by slower migration of the protein detected on Western blots, which could be eliminated by incubation of samples with alkaline phosphatase. A close correspondence was observed between the time- and dose-dependency of LPA effects on communication and the induction of mitogen-activated protein kinase (MAP kinase). Activation of both the 42 kDa and 44 kDa subspecies were confirmed by mobility shifts on Western blots using an anti-(MAP kinase R1) (erk 1-III) antibody and by fractionation on Mono Q columns. Cells pretreated with phorbol ester for 24 h were insensitive to phorbol ester inhibition of communication or activation of MAP kinase, but retained their sensitivity to LPA. The results indicate that LPA initiates the activation of protein kinase cascades in WB cells that are probably independent of protein kinase C and identifies connexin-43 as one substrate for the activated kinases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.