Evidence map›Paper›PMID 8182145›Full record

ArticleThe Journal of clinical investigation1994

Ligation of the T cell receptor complex results in activation of the Ras/Raf-1/MEK/MAPK cascade in human T lymphocytes.

R A Franklin, A Tordai, H Patel, A M Gardner, G L Johnson, E W Gelfand

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 114 citations in OpenAlex.

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  13. T-cell receptor signaling is mediated by transient Lck activity, which is inhibited by inorganic mercury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009
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  20. Activation of CD4 T cells by Raf-independent effectors of Ras.Proceedings of the National Academy of Sciences of the United States of America · 2003
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

R A FranklinDepartment of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
A Tordai
H Patel
A M Gardner
G L Johnson
E W Gelfand
University of Colorado Denver · USNational Jewish Health · US

Funding

REGULATION OF SEQUENTIAL PROTEIN KINASE PATHWAYSR01DK037871 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI JOHNSON, GARY L. · 1986 to 2011
$3.7M
MOLECULAR BASIS OF HIV INDUCED IMMUNE SUPPRESSIONP01AI029903 · NIAID · NATIONAL JEWISH HEALTH · PI CAMBIER, JOHN C. · 1990 to 1994
–
ACTIVATION &TRANSFORMATION OF HUMAN B CELLS BY EB VIRUSR01AI029704 · NIAID · NATIONAL JEWISH HEALTH · PI GELFAND, ERWIN WILLIAM · 1990 to 1992
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NIAID NIH HHS P01-AI29903NIAID NIH HHS R01-AI29704NIDDK NIH HHS DK37871
6 · The paper itself

Abstract

Stimulation of T cells with antibodies directed towards the T cell receptor complex results in the activation of mitogen-associated protein kinase (MAPK). Two pathways have been described in other cell types that can lead to MAPK activation. One of these pathways involves the activation of Ras, leading to the activation of Raf-1, and the subsequent activation of MEK (MAPK or ERK kinase). The contribution of this pathway in T cells for anti-CD3 or phorbol myristate acetate (PMA)-mediated MAPK activation was examined. We detected the kinase activities of Raf-1 and MEK towards their substrates (MEK for Raf-1 and MAPK for MEK) in this pathway leading to the activation of MAPK. Stimulation of the T cells with either anti-CD3 antibody or PMA resulted in a rapid activation of both Ras and Raf-1. MEK activity towards kinase-active or -inactive recombinant MAPK also increased upon stimulation. In addition, both MAPK and p90rsk were activated in these cells. We suggest that activation of MAPK and the subsequent activation of ribosomal S6 kinase (p90rsk) occurs by the Ras/Raf-1/MEK cascade in T lymphocytes stimulated by ligation of the T cell receptor complex.

Indexed as

Lymphocyte ActivationMitogen-Activated Protein Kinase KinasesSignal TransductionCalcium-Calmodulin-Dependent Protein KinasesCD3 ComplexCross-Linking ReagentsHumansMAP Kinase Kinase 1Models, BiologicalOncogene Protein p21(ras)Protein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-rafReceptors, Antigen, T-CellRibosomal Protein S6 KinasesCalcium-Calmodulin-Dependent Protein KinasesCD3 ComplexCross-Linking ReagentsMAP2K1 protein, humanMAP Kinase Kinase 1Mitogen-Activated Protein Kinase KinasesOncogene Protein p21(ras)Protein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-rafReceptors, Antigen, T-CellRibosomal Protein S6 KinasesTetradecanoylphorbol Acetate

Identifiers

PMID8182145
PMCPMC294346
OpenAlexW2041815456

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.