Evidence map›Paper›PMID 8254772›Full record

ArticleJournal of virology1994

Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type I basal gene expression.

F Kashanchi, J F Duvall, J Dittmer, A Mireskandari, R L Reid, S D Gitlin, J N Brady

Open access · bronzeAbstract read
In one paragraph

Article in Journal of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Review
  3. The Pleiotropic Effects of YBX1 on HTLV-1 Transcription.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

F KashanchiLaboratory of Molecular Virology, National Institutes of Health, Bethesda, Maryland 20892.
J F Duvall
J Dittmer
A Mireskandari
R L Reid
S D Gitlin
J N Brady
National Institutes of Health · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sequences which control basal human T-cell lymphotropic virus type I (HTLV-I) transcription likely play an important role in initiation and maintenance of virus replication. We previously identified and analyzed a 45-nucleotide sequence (downstream regulatory element 1 [DRE 1]), +195 to +240, at the boundary of the R/U5 region of the long terminal repeat which is required for HTLV-I basal transcription. We identified a protein, p37, which specifically bound to DRE 1. An affinity column fraction, containing p37, stimulated HTLV-I transcription approximately 12-fold in vitro. We now report the identification of a cDNA clone (15B-7), from a Jurkat expression library, that binds specifically to the DRE 1 regulatory sequence. Binding of the cDNA fusion protein, similarly to the results obtained with purified Jurkat protein, was decreased by introduction of site-specific mutations in the DRE 1 regulatory sequence. In vitro transcription and translation of 15B-7 cDNA produced a fusion protein which bound specifically to the HTLV-I +195 to +240 oligonucleotide. The partial cDNA encodes a protein which is homologous to the C-terminal 196 amino acids of the 36-kDa transcription factor, YB-1. Cotransfection of a YB-1 expression plasmid increases HTLV-I basal transcription approximately 14-fold in Jurkat T lymphocytes. On the basis of the molecular weight, DNA-binding characteristics, and in vivo transactivation activity, we suggest that the previously identified DRE 1-binding protein, p37, is YB-1.

Indexed as

CCAAT-Enhancer-Binding ProteinsGene Expression Regulation, ViralAmino Acid SequenceBase SequenceCell LineCell Transformation, ViralChloramphenicol O-AcetyltransferaseDNA-Binding ProteinsHumansHuman T-lymphotropic virus 1Molecular Sequence DataNFI Transcription FactorsNuclear ProteinsRecombinant Fusion ProteinsRegulatory Sequences, Nucleic AcidRNA, MessengerCCAAT-Enhancer-Binding ProteinsChloramphenicol O-AcetyltransferaseDNA-Binding ProteinsNFI Transcription FactorsNuclear ProteinsRecombinant Fusion ProteinsRNA, MessengerTranscription FactorsY-Box-Binding Protein 1YBX1 protein, human

Identifiers

PMID8254772
PMCPMC236322
OpenAlexW1589428890

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.