ArticleMolecular and cellular biology1993
An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Systematic identification of Oct4 transcriptional targets in embryonic stem cells using the auxin-inducible degron system and nascent RNA sequencing.Cell regeneration (London, England) · 2025Article
- Retinoic Acid Receptor β Loss in Hepatocytes Increases Steatosis and Elevates the Integrated Stress Response in Alcohol-Associated Liver Disease.International journal of molecular sciences · 2023Article
- miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming.Stem cell research & therapy · 2017Article
- Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment.In vitro cellular & developmental biology. Animal · 2016Article
- Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells.Stem cells and development · 2016Article
- Current concept in neural regeneration research: NSCs isolation, characterization and transplantation in various neurodegenerative diseases and stroke: A review.Journal of advanced research · 2014Review
- Review
- Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression.Developmental biology · 2011Article
- Rex1/Zfp42 as an epigenetic regulator for genomic imprinting.Human molecular genetics · 2011Article
- Retinoids regulate stem cell differentiation.Journal of cellular physiology · 2011Review
- Transcriptional regulation of Rex1 (zfp42) in normal prostate epithelial cells and prostate cancer cells.Journal of cellular physiology · 2010Article
- Stem cell pluripotency: a cellular trait that depends on transcription factors, chromatin state and a checkpoint deficient cell cycle.Journal of cellular physiology · 2009Review
- Regulation of stem cell pluripotency and differentiation involves a mutual regulatory circuit of the NANOG, OCT4, and SOX2 pluripotency transcription factors with polycomb repressive complexes and stem cell microRNAs.Stem cells and development · 2009Review
- Dax1 binds to Oct3/4 and inhibits its transcriptional activity in embryonic stem cells.Molecular and cellular biology · 2009Article
- Analysis of Rex1 (zfp42) function in embryonic stem cell differentiation.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- Article
- Cross-species de novo identification of cis-regulatory modules with GibbsModule: application to gene regulation in embryonic stem cells.Genome research · 2008Article
- Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter.PloS one · 2008Article
- Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.BMC developmental biology · 2008Article
- Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-freeBMP4 culture.BMC genomics · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.