ArticleMolecular and cellular biology1996
Raf, but not MEK or ERK, is sufficient for differentiation of hippocampal neuronal cells.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 87 citations in OpenAlex.
- Article
- cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells.Scientific reports · 2017Article
- Nuclear Raf-1 kinase regulates the CXCR5 promoter by associating with NFATc3 to drive retinoic acid-induced leukemic cell differentiation.The FEBS journal · 2014Article
- Phospholipase D1 is an important regulator of bFGF-induced neurotrophin-3 expression and neurite outgrowth in H19-7 cells.Molecular neurobiology · 2012Article
- Regulation of cellular proliferation, differentiation and cell death by activated Raf.Cell communication and signaling : CCS · 2009Article
- Cytosolic PLA2 is required for CTL-mediated immunopathology of celiac disease via NKG2D and IL-15.The Journal of experimental medicine · 2009Article
- Vitamin D and differentiation in cancer.Critical reviews in clinical laboratory sciences · 2009Review
- Raf-1 signaling is required for the later stages of 1,25-dihydroxyvitamin D3-induced differentiation of HL60 cells but is not mediated by the MEK/ERK module.Journal of cellular physiology · 2006Article
- The EphA8 receptor induces sustained MAP kinase activation to promote neurite outgrowth in neuronal cells.Oncogene · 2005Article
- Differentiation of neuroblastoma cell line N1E-115 involves several signaling cascades.Neurochemical research · 2005Article
- A Raf-1 mutant that dissociates MEK/extracellular signal-regulated kinase activation from malignant transformation and differentiation but not proliferation.Molecular and cellular biology · 2003Article
- AMP-activated protein kinase is highly expressed in neurons in the developing rat brain and promotes neuronal survival following glucose deprivation.Journal of molecular neuroscience : MN · 2001Article
- Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- MEK kinase activity is not necessary for Raf-1 function.The EMBO journal · 2001Article
- A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity.Molecular and cellular biology · 2001Article
- Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions.The Biochemical journal · 2000Review
- Different protein kinase C isoforms determine growth factor specificity in neuronal cells.Molecular and cellular biology · 2000Article
- Article
- Protein kinase Cdelta mediates neurogenic but not mitogenic activation of mitogen-activated protein kinase in neuronal cells.Molecular and cellular biology · 1999Article
- Estrogen-induced activation of mitogen-activated protein kinase in cerebral cortical explants: convergence of estrogen and neurotrophin signaling pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
To elucidate signal transduction pathways leading to neuronal differentiation, we have investigated a conditionally immortalized cell line from rat hippocampal neurons (H19-7) that express a temperature sensitive simian virus 40 large T antigen. Treatment of H19-7 cells with the differentiating agent basic fibroblast growth factor at 39 degrees C, the nonpermissive temperature for T function, resulted in the activation of c-Raf-1, MEK, and mitogen-activated protein (MAP) kinases (ERK1 and -2). To evaluate the role of Raf-1 in neuronal cell differentiation, we stably transfected H19-7 cells with v-raf or an oncogenic human Raf-1-estrogen receptor fusion gene (deltaRaf-1:ER). deltaRaf-1:ER transfectants in the presence of estradiol for 1 to 2 days expressed a differentiation phenotype only at the nonpermissive temperature. However, extended exposure of the deltaRaf-1:ER transfectants to estradiol or stable expression of the v-raf construct yielded cells that extended processes at the permissive as well as the nonpermissive temperature, suggesting that cells expressing the large T antigen are capable of responding to the Raf differentiation signal. deltaRaf-1:ER, MEK, and MAP kinase activities in the deltaRaf-1:ER cells were elevated constitutively for up to 36 h of estradiol treatment at the permissive temperature. At the nonpermissive temperature, MEK and ERKs were activated to a significantly lesser extent, suggesting that prolonged MAP kinase activation may not be sufficient for differentiation. To test this possibility, H19-7 cells were transfected or microinjected with constitutively activated MEK. The results indicate that prolonged activation of MEK or MAP kinases (ERK1 and -2) is not sufficient for differentiation of H19-7 neuronal cells and raise the possibility that an alternative signaling pathway is required for differentiation of H19-7 cells by Raf.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.