ArticleMolecular and cellular biology1996
Activation of codependent transcription factors is required for transcriptional induction of the vgf gene by nerve growth factor and Ras.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Grape-derived polyphenols produce antidepressant effects via VGF- and BDNF-dependent mechanisms.Annals of the New York Academy of Sciences · 2019Article
- Role for Egr1 in the Transcriptional Program Associated with Neuronal Differentiation of PC12 Cells.PloS one · 2017Article
- NPAS3 Regulates Transcription and Expression of VGF: Implications for Neurogenesis and Psychiatric Disorders.Frontiers in molecular neuroscience · 2016Article
- Neuroendocrine Role for VGF.Frontiers in endocrinology · 2015Review
- Trk retrograde signaling requires persistent, Pincher-directed endosomes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Neuropeptides in depression: role of VGF.Behavioural brain research · 2009Review
- PLCgamma2 Activates CREB-dependent Transcription in PC12 Cells Through Phosphorylation of CREB at Serine 133.Cytotechnology · 2005Article
- Processing, distribution, and function of VGF, a neuronal and endocrine peptide precursor.Cellular and molecular neurobiology · 2004Review
- Temporal characteristics of activation, deactivation, and restimulation of signal transduction following depolarization in the pheochromocytoma cell line PC12.Molecular and cellular biology · 2003Article
- VGF is required for obesity induced by diet, gold thioglucose treatment, and agouti and is differentially regulated in pro-opiomelanocortin- and neuropeptide Y-containing arcuate neurons in response to fasting.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Sustained signaling by phospholipase C-gamma mediates nerve growth factor-triggered gene expression.Molecular and cellular biology · 2001Article
- Nerve growth factor, but not epidermal growth factor, increases Fra-2 expression and alters Fra-2/JunD binding to AP-1 and CREB binding elements in pheochromocytoma (PC12) cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Regulation of tumor growth and metastasis of human melanoma by the CREB transcription factor family.Molecular and cellular biochemistry · 2000Article
- Tissue- and development-specific expression of multiple alternatively spliced transcripts of rat neuronal nitric oxide synthase.The Journal of clinical investigation · 1997Article
- Role of the bZIP transcription factor IREBF1 in the NGF induction of stromelysin-1 (transin) gene expression in PC12 cells.Journal of molecular neuroscience : MN · 1997Article
- Regulation of the neural-specific gene VGF in PC12 cells. Identification of transcription factors interacting with NGF-responsive elements.Journal of molecular neuroscience : MN · 1997Article
- Interplay of the E box, the cyclic AMP response element, and HTF4/HEB in transcriptional regulation of the neurospecific, neurotrophin-inducible vgf gene.Molecular and cellular biology · 1997Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nerve growth factor (NGF) treatment of PC12 cells leads to the elaboration of a neuronal phenotype, including the induction of neuronally expressed genes such as vgf. To study vgf transcription, we have created chimeric vgf/beta-globin genes in which vgf promoter sequences drive the expression of the beta-globin reporter gene or of a chimeric beta-globin gene fused to 3' untranslated vgf gene sequences. We have found that the level of inducibility of the latter construct by NGF resembles that of the endogenous vgf gene. Using transient transfection of the chimeric reporter genes into PC12 cells, into PC12 subclones expressing activated or dominantly interfering mutant Ras proteins, and into PC12 variants expressing specific NGF receptor/Trk mutants, we show that transcriptional regulation of the vgf promoter by NGF is mediated through a Ras-dependent signaling pathway. By mutational analysis of the vgf promoter, we have identified three promoter elements involved in mediating transcriptional induction by NGF and Ras. In addition to the cyclic AMP-responsive element (CRE), which binds to ATF-1, ATF-2, and CRE-binding protein in PC12 nuclear extracts, a novel CCAAT element and its binding proteins were identified, which, like the CRE, is necessary but not sufficient for the Ras-dependent induction of the vgf gene by NGF. We also identify a G(S)G element unusually located between the TATA box and transcriptional start site, which binds the NGF- and Ras-induced transcription factor, NGFI-A, and amplifies the transcriptional response. Integrating data from studies of vgf promoter regulation and NGF signal transduction, we present a model for vgf gene induction in which transcriptional activation is achieved through the persistent, direct activation of multiple interacting transcription factors binding to CRE and CCAAT elements, coordinated with the delayed transcription factor action at a G(S)G element resulting from the induced expression of NGFI-A.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.