ArticlePlant molecular biology1996
Alloplasmic male-sterile Brassica lines containing B. tournefortii mitochondria express an ORF 3' of the atp6 gene and a 32 kDa protein. off.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review and Developmental Perspective on Origin of CMS Genes in Crops.International journal of molecular sciences · 2024Pooled it
- Article
- A breeding method for Ogura CMS restorer line independent of restorer source inFrontiers in genetics · 2024Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Comparative analysis of mitochondrial genomes of two alpine medicinal plants of Gentiana (Gentianaceae).PloS one · 2023Article
- Transcriptomic and iTRAQ-Based Quantitative Proteomic Analyses ofPlants (Basel, Switzerland) · 2022Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Complete mitochondrial genome sequencing and identification of candidate genes responsible for C5-type cytoplasmic male sterility in cabbage (Frontiers in plant science · 2022Article
- Mitochondrial genes are involved in the fertility transformation of the thermosensitive male-sterile line YS3038 in wheat.Molecular breeding : new strategies in plant improvement · 2021Article
- Elucidating Mitochondrial DNA Markers ofFrontiers in plant science · 2021Article
- Comparative Analysis of the Mitochondrial Genomes ofFrontiers in genetics · 2020Article
- Organelle genome composition and candidate gene identification for Nsa cytoplasmic male sterility in Brassica napus.BMC genomics · 2019Article
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- Development of KASP marker for cytoplasmic male sterility inJournal of genetics · 2019Article
- Mitochondrial genome and transcriptome analysis of five alloplasmic male-sterile lines in Brassica juncea.BMC genomics · 2019Article
- Article
- A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence.Plant molecular biology · 2018Article
- Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea.Journal of experimental botany · 2018Article
- Morphological and genetic characterization of a new cytoplasmic male sterility system (oxa CMS) in stem mustard (Brassica juncea).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- A Novel Cytoplasmic Male Sterility inFrontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyses of mitochondrial transcription and in organello translation were performed with the Brassica tournefortii cytoplasm. This cytoplasm causes alloplasmic male sterility when combined with the nuclear genomes of B. napus and B. juncea. Mitochondrial RNA and protein banding patterns were compared between the fertile wild species B. tournefortii, an alloplasmic male-sterile B. juncea line, an alloplasmic male-sterile B. napus line and an alloplasmic B. napus line with restored fertility. The analyses were carried out to identify differences in gene expression and to investigate whether alterations in gene expression accompanied male sterility. A difference in transcription patterns between the fertile B. tournefortii and the alloplasmic lines was found for the atp6 gene. The atp6 region was investigated further, since a similar alteration in atp6 transcription has been observed in two other Brassica cytoplasms which are associated with cytoplasmic male sterility (CMS). The additional longer atp6 transcript detected in the alloplasmic lines in the present study was found to contain an open reading frame (ORF) located downstream of the atp6 gene. DNA sequencing revealed that the ORF, orf263, could encode a protein with a predicted molecular weight of about 29 kDa. In organello analysis detected two proteins of 29 and 32 kDa respectively, which were found only in the alloplasmic lines. Furthermore, the 32 kDa protein accompanied male sterility since it was absent in alloplasmic plants restored to fertility. The protein analysis might indicate that orf263 is translated and causes CMS.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.