ArticleThe Journal of cell biology1997
MAP kinase is required for the spindle assembly checkpoint but is dispensable for the normal M phase entry and exit in Xenopus egg cell cycle extracts.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 111 citations in OpenAlex.
- Article
- MAPK-dependent control of mitotic progression in S. pombe.BMC biology · 2024Article
- Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis.Frontiers in cell and developmental biology · 2024Review
- The Ovarian Development Genes of Bisexual and ParthenogeneticFrontiers in veterinary science · 2021Article
- Article
- Conserved insulin signaling in the regulation of oocyte growth, development, and maturation.Molecular reproduction and development · 2017Review
- Making an effective switch at the kinetochore by phosphorylation and dephosphorylation.Chromosoma · 2013Review
- Transcriptome profiling of testis during sexual maturation stages in Eriocheir sinensis using Illumina sequencing.PloS one · 2012Article
- Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation.Cellular & molecular biology letters · 2011Article
- Mitogen-activated protein/extracellular signal-regulated kinase kinase 1act/tubulin interaction is an important determinant of mitotic stability in cultured HT1080 human fibrosarcoma cells.Cancer research · 2010Article
- A novel role for Cdk1/cyclin B in regulating B-raf activation at mitosis.Molecular biology of the cell · 2008Article
- Roles of Greatwall kinase in the regulation of cdc25 phosphatase.Molecular biology of the cell · 2008Article
- Mitogen-activated protein kinase kinase 1-dependent Golgi unlinking occurs in G2 phase and promotes the G2/M cell cycle transition.Molecular biology of the cell · 2007Article
- MAP kinase meets mitosis: a role for Raf Kinase Inhibitory Protein in spindle checkpoint regulation.Cell division · 2007Article
- Extracellular signal-regulated kinase 1/2 activity is not required in mammalian cells during late G2 for timely entry into or exit from mitosis.Molecular biology of the cell · 2006Article
- Raf kinase inhibitory protein regulates aurora B kinase and the spindle checkpoint.Molecular cell · 2006Article
- Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.Molecular biology of the cell · 2005Article
- NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis.Genes & development · 2003Article
- Distinct cell cycle timing requirements for extracellular signal-regulated kinase and phosphoinositide 3-kinase signaling pathways in somatic cell mitosis.Molecular and cellular biology · 2002Article
- Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Xenopus laevis egg cell cycle extracts that mimic early embryonic cell cycles, activation of MAP kinase and MAP kinase kinase occurs in M phase, slightly behind that of maturation promoting factor. To examine the possible role of MAP kinase in the in vitro cell cycle, we depleted the extracts of MAP kinase by using anti-Xenopus MAP kinase antibody. Like in the mock-treated extracts, the periodic activation and deactivation of MPF occurred normally in the MAP kinase-depleted extracts, suggesting that MAP kinase is dispensable for the normal M phase entry and exit in vitro. It has recently been reported that microtubule depolymerization by nocodazole treatment can block exit from mitosis in the extracts if enough sperm nuclei are present, and that the addition of MAP kinase-specific phosphatase MKP-1 overcomes this spindle assembly checkpoint, suggesting the involvement of MAP kinase in the checkpoint signal transduction. We show here that the spindle assembly checkpoint mechanism cannot operate in the MAP kinase-depleted extracts. But, adding recombinant Xenopus MAP kinase to the MAP kinase-depleted extracts restored the spindle assembly checkpoint. These results indicate unambiguously that classical MAP kinase is required for the spindle assembly checkpoint in the cell cycle extracts. In addition, we show that strong activation of MAP kinase by the addition of a constitutively active MAP kinase kinase kinase in the absence of sperm nuclei and nocodazole, induced mitotic arrest in the extracts. Therefore, activation of MAP kinase alone is sufficient for inducing the mitotic arrest in vitro.
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