ArticleThe Journal of cell biology1997
A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 110 citations in OpenAlex.
- MAPK-dependent control of mitotic progression in S. pombe.BMC biology · 2024Article
- Role of spindle assembly checkpoint proteins in gametogenesis and embryogenesis.Frontiers in cell and developmental biology · 2024Review
- H2S-releasing nanoemulsions: a new formulation to inhibit tumor cells proliferation and improve tissue repair.Oncotarget · 2016Article
- Making an effective switch at the kinetochore by phosphorylation and dephosphorylation.Chromosoma · 2013Review
- CpG Island Methylation, Microsatellite Instability, and BRAF Mutations and Their Clinical Application in the Treatment of Colon Cancer.Chemotherapy research and practice · 2012Article
- The roles of cyclin A2, B1, and B2 in early and late mitotic events.Molecular biology of the cell · 2010Article
- A novel role for Cdk1/cyclin B in regulating B-raf activation at mitosis.Molecular biology of the cell · 2008Article
- MAP kinase meets mitosis: a role for Raf Kinase Inhibitory Protein in spindle checkpoint regulation.Cell division · 2007Article
- Extracellular signal-regulated kinase 1/2 activity is not required in mammalian cells during late G2 for timely entry into or exit from mitosis.Molecular biology of the cell · 2006Article
- Raf kinase inhibitory protein regulates aurora B kinase and the spindle checkpoint.Molecular cell · 2006Article
- Inhibiting MAP kinase activity prevents calcium transients and mitosis entry in early sea urchin embryos.The Journal of biological chemistry · 2005Article
- Phosphorylation and activation of Bub1 on unattached chromosomes facilitate the spindle checkpoint.The EMBO journal · 2004Article
- The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Review
- Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest.The Journal of cell biology · 2003Article
- NQK1/NtMEK1 is a MAPKK that acts in the NPK1 MAPKKK-mediated MAPK cascade and is required for plant cytokinesis.Genes & development · 2003Article
- Distinct cell cycle timing requirements for extracellular signal-regulated kinase and phosphoinositide 3-kinase signaling pathways in somatic cell mitosis.Molecular and cellular biology · 2002Article
- Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes.Molecular biology of the cell · 2002Article
- Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity.The Journal of cell biology · 2001Article
- A specific activation of the mitogen-activated protein kinase kinase 1 (MEK1) is required for Golgi fragmentation during mitosis.The Journal of cell biology · 2000Article
- Activation of Wee1 by p42 MAPK in vitro and in cycling xenopus egg extracts.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The spindle assembly checkpoint prevents cells whose spindles are defective or chromosomes are misaligned from initiating anaphase and leaving mitosis. Studies of Xenopus egg extracts have implicated the Erk2 mitogen-activated protein kinase (MAP kinase) in this checkpoint. Other studies have suggested that MAP kinases might be important for normal mitotic progression. Here we have investigated whether MAP kinase function is required for mitotic progression or the spindle assembly checkpoint in vivo in Xenopus tadpole cells (XTC). We determined that Erk1 and/or Erk2 are present in the mitotic spindle during prometaphase and metaphase, consistent with the idea that MAP kinase might regulate or monitor the status of the spindle. Next, we microinjected purified recombinant XCL100, a Xenopus MAP kinase phosphatase, into XTC cells in various stages of mitosis to interfere with MAP kinase activation. We found that mitotic progression was unaffected by the phosphatase. However, XCL100 rendered the cells unable to remain arrested in mitosis after treatment with nocodazole. Cells injected with phosphatase at prometaphase or metaphase exited mitosis in the presence of nocodazole-the chromosomes decondensed and the nuclear envelope re-formed-whereas cells injected with buffer or a catalytically inactive XCL100 mutant protein remained arrested in mitosis. Coinjection of constitutively active MAP kinase kinase-1, which opposes XCL100's effects on MAP kinase, antagonized the effects of XCL100. Since the only known targets of MAP kinase kinase-1 are Erk1 and Erk2, these findings argue that MAP kinase function is required for the spindle assembly checkpoint in XTC cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.