ArticleMolecular and cellular biology1997
Differentiation of central nervous system neuronal cells by fibroblast-derived growth factor requires at least two signaling pathways: roles for Ras and Src.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 79 citations in OpenAlex.
- Article
- Expression of a novel brain specific isoform of C3G is regulated during development.Scientific reports · 2020Article
- The role of SRC kinase in the caspase-1 pathway after hypoxia in the brain of newborn piglets.Neurochemical research · 2014Article
- Neuronal vs. glial fate of embryonic stem cell-derived neural progenitors (ES-NPs) is determined by FGF2/EGF during proliferation.Journal of molecular neuroscience : MN · 2010Article
- Kinase/phosphatase overexpression reveals pathways regulating hippocampal neuron morphology.Molecular systems biology · 2010Article
- SRC family kinases are required for limb trajectory selection by spinal motor axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Activation of Src-family kinases in spinal microglia contributes to mechanical hypersensitivity after nerve injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Role of Cdc42 in neurite outgrowth of PC12 cells and cerebellar granule neurons.Molecular and cellular biochemistry · 2006Article
- Mediation of basic fibroblast growth factor-induced lactotropic cell proliferation by Src-Ras-mitogen-activated protein kinase p44/42 signaling.Endocrinology · 2005Article
- Tyrosine phosphorylation of Sprouty proteins regulates their ability to inhibit growth factor signaling: a dual feedback loop.Molecular biology of the cell · 2004Article
- Analysis of the PC12 cell transcriptome after differentiation with pituitary adenylate cyclase-activating polypeptide (PACAP).Journal of neurochemistry · 2002Article
- A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity.Molecular and cellular biology · 2001Article
- Different protein kinase C isoforms determine growth factor specificity in neuronal cells.Molecular and cellular biology · 2000Article
- Mapping of atypical protein kinase C within the nerve growth factor signaling cascade: relationship to differentiation and survival of PC12 cells.Molecular and cellular biology · 2000Article
- Contribution of Src and Ras pathways in FGF-2 induced endothelial cell differentiation.Oncogene · 1999Article
- Protein kinase Cdelta mediates neurogenic but not mitogenic activation of mitogen-activated protein kinase in neuronal cells.Molecular and cellular biology · 1999Article
- Raf and fibroblast growth factor phosphorylate Elk1 and activate the serum response element of the immediate early gene pip92 by mitogen-activated protein kinase-independent as well as -dependent signaling pathways.Molecular and cellular biology · 1998Article
- Akt, a target of phosphatidylinositol 3-kinase, inhibits apoptosis in a differentiating neuronal cell line.Molecular and cellular biology · 1998Article
- Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
To evaluate the role of mitogen-activated protein (MAP) kinase and other signaling pathways in neuronal cell differentiation by basic fibroblast-derived growth factor (bFGF), we used a conditionally immortalized cell line from rat hippocampal neurons (H19-7). Previous studies have shown that activation of MAP kinase kinase (MEK) is insufficient to induce neuronal differentiation of H19-7 cells. To test the requirement for MEK and MAP kinase (ERK1 and ERK2), H19-7 cells were treated with the MEK inhibitor PD098059. Although the MEK inhibitor blocked the induction of differentiation by constitutively activated Raf, the H19-7 cells still underwent differentiation by bFGF. These results suggest that an alternative pathway is utilized by bFGF for differentiation of the hippocampal neuronal cells. Expression in the H19-7 cells of a dominant-negative Ras (N17-Ras) or Raf (C4-Raf) blocked differentiation by bFGF, suggesting that Ras and probably Raf are required. Expression of dominant-negative Src (pcSrc295Arg) or microinjection of an anti-Src antibody blocked differentiation by bFGF in H19-7 cells, indicating that bFGF also signals through a Src kinase-mediated pathway. Although neither constitutively activated MEK (MEK-2E) nor v-Src was sufficient individually to differentiate the H19-7 cells, coexpression of constitutively activated MEK and v-Src induced neurite outgrowth. These results suggest that (i) activation of MAP kinase (ERK1 and ERK2) is neither necessary nor sufficient for differentiation by bFGF; (ii) activation of Src kinases is necessary but not sufficient for differentiation by bFGF; and (iii) differentiation of H19-7 neuronal cells by bFGF requires at least two signaling pathways activated by Ras and Src.
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