Evidence mapPaperPMID 9276741Full record

ArticleThe Journal of clinical investigation1997

Elevated circulating free fatty acid levels impair endothelium-dependent vasodilation.

H O Steinberg, M Tarshoby, R Monestel, G Hook, J Cronin, A Johnson, B Bayazeed, A D Baron

Registry-linked trialOpen access · bronzeAbstract read
In one paragraph

Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02633488 (Effect of Metformin on Insulin Sensitivity and Pan-Arterial Vascular Function in Adults With Metabolic Syndrome), which is not on this map. Cited by 206 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
206citing papers in PubMed, 2 pooled it
11.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02633488 nacompletedstarted 2014, after this paper: background citation

Effect of Metformin on Insulin Sensitivity and Pan-Arterial Vascular Function in Adults With Metabolic Syndrome

Ran2014Enrolled19Registered outcomes1Posted comparisons1ConditionsInsulin Sensitivity, Metabolic SyndromeArmsMetformin, Placebos
Open the trial in the graph
3 · Its place in the literature

Who cites it

206 citing papers in PubMed, 2 syntheses or guidelines pooled it, 760 citations in OpenAlex.

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  12. Free fatty acids induce insulin resistance in both cardiac and skeletal muscle microvasculature in humans.The Journal of clinical endocrinology and metabolism · 2011 · on this map
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  19. Endothelial Metabolic Reprogramming Links Diabetes to Atherosclerosis.Diabetes, metabolic syndrome and obesity : targets and therapy · 2026
    Review
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146 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

H O SteinbergDepartment of Medicine, Indiana University Medical Center, Indianapolis, Indiana 46202, USA.
M Tarshoby
R Monestel
G Hook
J Cronin
A Johnson
B Bayazeed
A D Baron
University Medical Center · USIndiana University – Purdue University Indianapolis · US

Funding

ROLE OF HEMODYNAMICS IN IN VIVO INSULIN RESISTANCER37DK042469 · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · 1999 to 2003
$1.1M
ROLE OF HEMODYNAMICS IN IN VIVO INSULIN RESISTANCER01DK042469 · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · 1995 to 1998
ROLE OF HEMODYNAMICS IN IN VIVO INSULIN RESISTANCER29DK042469 · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · 1990 to 1994
NCRR NIH HHS MO1-RR750-19NIDDK NIH HHS DK20452NIDDK NIH HHS DK42469
6 · The paper itself

Abstract

We have recently shown that insulin-resistant obese subjects exhibit impaired endothelial function. Here, we test the hypothesis that elevation of circulating FFA to levels seen in insulin-resistant subjects can impair endothelial function. We studied leg blood flow responses to graded intrafemoral artery infusions of the endothelium-dependent vasodilator methacholine chloride (Mch) or the endothelium-independent vasodilator sodium nitroprusside during the infusion of saline and after raising systemic circulating FFA levels exogenously via a low- or high-dose infusion of Intralipid plus heparin or endogenously by an infusion of somatostatin (SRIF) to produce insulinopenia in groups of lean healthy humans. After 2 h of infusion of Intralipid plus heparin, FFA levels increased from 562+/-95 to 1,303+/-188 micromol, and from 350+/-35 to 3,850+/-371 micromol (P < 0.001) vs. saline for both low- and high-dose groups, respectively. Mch-induced vasodilation relative to baseline was reduced by approximately 20% in response to the raised FFA levels in both groups (P < 0.05, saline vs. FFA, ANOVA). In contrast, similar FFA elevation did not change leg blood flow responses to sodium nitroprusside. During the 2-h SRIF infusion, insulin levels fell, and FFA levels rose from 474+/-22 to 1,042+/-116 micromol (P < 0.01); Mch-induced vasodilation was reduced by approximately 20% (P < 0.02, saline vs. SRIF, ANOVA). Replacement of basal insulin levels during SRIF resulted in a fall of FFA levels from 545+/-47 to 228+/-61 micromol, and prevented the impairment of Mch-induced vasodilation seen with SRIF alone. In conclusion, (a) elevated circulating FFA levels cause endothelial dysfunction, and (b) impaired endothelial function in insulin-resistant humans may be secondary to the elevated FFA concentrations observed in these patients.

Indexed as

VasodilationAdultBlood PressureEndothelium, VascularFat Emulsions, IntravenousFatty Acids, NonesterifiedFemaleHumansInsulinLegMaleMethacholine ChlorideNitroprussideSomatostatinFat Emulsions, IntravenousFatty Acids, NonesterifiedInsulinMethacholine ChlorideNitroprussideSomatostatin

Identifiers

PMID9276741
PMCPMC508300
OpenAlexW2086785365

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.