ArticleThe Journal of clinical investigation1997
Tissue- and development-specific expression of multiple alternatively spliced transcripts of rat neuronal nitric oxide synthase.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 82 citations in OpenAlex.
- Nitric oxide regulates the firing rate of neuronal subtypes in the guinea pig ventral cochlear nucleus.The European journal of neuroscience · 2020Review
- CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts.PLoS genetics · 2019Article
- Inhibition of Nitric Oxide Synthase 1 Induces Salt-Sensitive Hypertension in Nitric Oxide Synthase 1α Knockout and Wild-Type Mice.Hypertension (Dallas, Tex. : 1979) · 2016Article
- Nuclear recruitment of neuronal nitric-oxide synthase by α-syntrophin is crucial for the induction of mitochondrial biogenesis.The Journal of biological chemistry · 2014Article
- Cyclic AMP-dependent phosphorylation of neuronal nitric oxide synthase mediates penile erection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Renal nitric oxide production in rat pregnancy: role of constitutive nitric oxide synthases.American journal of physiology. Renal physiology · 2010Article
- Salt-sensitive splice variant of nNOS expressed in the macula densa cells.American journal of physiology. Renal physiology · 2010Article
- Splice variants of neuronal nitric oxide synthase are present in the rat kidney.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2009Article
- Renal cortex neuronal nitric oxide synthase in response to rapamycin in kidney transplantation.Nitric oxide : biology and chemistry · 2008Article
- Coregulation of natively expressed pertussis toxin-sensitive muscarinic receptors with G-protein-activated potassium channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Alternatively spliced neuronal nitric oxide synthase mediates penile erection.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Hypoxia induces a functionally significant and translationally efficient neuronal NO synthase mRNA variant.The Journal of clinical investigation · 2005Article
- Multiple variable first exons: a mechanism for cell- and tissue-specific gene regulation.Genome research · 2004Article
- Nitric oxide is an essential negative regulator of cell proliferation in Xenopus brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- RNA diversity has profound effects on the translation of neuronal nitric oxide synthase.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Estrogen downregulates neuronal nitric oxide synthase in rat anterior pituitary cells and GH3 tumors.Endocrine · 1999Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Nitric oxide (NO) functions as an intercellular messenger and mediates numerous biological functions. Among the three isoforms of NO synthase that produce NO, the ubiquitously expressed neuronal NO synthase (nNOS) is responsible for a large part of NO production, yet its regulation is poorly understood. Recent reports of two alternative spliceforms of nNOS in the mouse and in man have raised the possibility of spatial and temporal modulation of expression. This study demonstrates the existence of at least three transcripts of the rat nNOS gene designated nNOSa, nNOSb, and nNOSc, respectively, with distinct 5' untranslated first exons that arise from alternative splicing to a common second exon. Expression of the alternative transcripts occurs with a high degree of tissue and developmental specificity, as demonstrated by RNase protection assays on multiple tissues from both fetal and adult rats. Furthermore, terminal differentiation of rat pheochromocytoma-derived PC12 cells into neurons is associated with induction of nNOSa, suggesting, likewise, development- and tissue-specific transcriptional control of nNOS isoform expression. Physical mapping using a rat yeast artificial chromosome clone shows that the alternatively spliced first exons 1a, 1b, and 1c are separated by at least 15-60 kb from the downstream coding sequence, with exons 1b and 1c being positioned within 200 bp of each other. These findings provide evidence that the biological activity of nNOS is tightly and specifically regulated by a complex pattern of alternative splicing, indicating that the notion of constitutive expression of this isoform needs to be revised.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.