ArticleMolecular and cellular biology1998
Characterization of a Rac1- and RhoGDI-associated lipid kinase signaling complex.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 144 citations in OpenAlex.
- A RAC1 SUMOylation switch governs breast cancer metastasis.Signal transduction and targeted therapy · 2026Article
- Underappreciated roles for Rho GDP dissociation inhibitors (RhoGDIs) in cell function: Lessons learned from the pancreatic islet β-cell.Biochemical pharmacology · 2022Review
- Subcellular Localization Relevance and Cancer-Associated Mechanisms of Diacylglycerol Kinases.International journal of molecular sciences · 2020Review
- The Volume-Regulated Anion Channel in Glioblastoma.Cancers · 2019Review
- Phosphatidylinositol Kinases and Phosphatases inFrontiers in cellular and infection microbiology · 2019Review
- DGK-θ: Structure, Enzymology, and Physiological Roles.Frontiers in cell and developmental biology · 2016Review
- Article
- Identification of key signaling molecules involved in the activation of the swelling-activated chloride current in human glioblastoma cells.The Journal of membrane biology · 2014Article
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Rac1 acts in conjunction with Nedd4 and dishevelled-1 to promote maturation of cell-cell contacts.Journal of cell science · 2012Article
- Comparison of the neutrophil proteome in trauma patients and normal controls.Protein and peptide letters · 2012Article
- GTP-binding protein-like domain of AGAP1 is protein binding site that allosterically regulates ArfGAP protein catalytic activity.The Journal of biological chemistry · 2012Article
- Localisation and composition of skeletal muscle diacylglycerol predicts insulin resistance in humans.Diabetologia · 2012Article
- Differential involvement of ezrin/radixin/moesin proteins in sphingosine 1-phosphate-induced human pulmonary endothelial cell barrier enhancement.Cellular signalling · 2011Article
- Coronin 1A promotes a cytoskeletal-based feedback loop that facilitates Rac1 translocation and activation.The EMBO journal · 2011Article
- Combined phosphoinositide and Ca2+ signals mediating receptor specificity toward neuronal Ca2+ channels.The Journal of biological chemistry · 2011Article
- One lipid, multiple functions: how various pools of PI(4,5)P(2) are created in the plasma membrane.Cellular and molecular life sciences : CMLS · 2010Review
- SUMOylation of the GTPase Rac1 is required for optimal cell migration.Nature cell biology · 2010Article
- Type I PIPK-alpha regulates directed cell migration by modulating Rac1 plasma membrane targeting and activation.The Journal of cell biology · 2010Article
- Lipid second messengers and related enzymes in vertebrate rod outer segments.Journal of lipid research · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
Rho family GTPases regulate a number of cellular processes, including actin cytoskeletal organization, cellular proliferation, and NADPH oxidase activation. The mechanisms by which these G proteins mediate their effects are unclear, although a number of downstream targets have been identified. The interaction of most of these target proteins with Rho GTPases is GTP dependent and requires the effector domain. The activation of the NADPH oxidase also depends on the C terminus of Rac, but no effector molecules that bind to this region have yet been identified. We previously showed that Rac interacts with a type I phosphatidylinositol-4-phosphate (PtdInsP) 5-kinase, independent of GTP. Here we report the identification of a diacylglycerol kinase (DGK) which also associates with both GTP- and GDP-bound Rac1. In vitro binding analysis using chimeric proteins, peptides, and a truncation mutant demonstrated that the C terminus of Rac is necessary and sufficient for binding to both lipid kinases. The Rac-associated PtdInsP 5-kinase and DGK copurify by liquid chromatography, suggesting that they bind as a complex to Rac. RhoGDI also associates with this lipid kinase complex both in vivo and in vitro, primarily via its interaction with Rac. The interaction between Rac and the lipid kinases was enhanced by specific phospholipids, indicating a possible mechanism of regulation in vivo. Given that the products of the PtdInsP 5-kinase and the DGK have been implicated in several Rac-regulated processes, and they bind to the Rac C terminus, these lipid kinases may play important roles in Rac activation of the NADPH oxidase, actin polymerization, and other signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.