Evidence map›Paper›PMID 9484647›Full record

ArticleInflammation1998

An apparently novel protein of human leukocytes, reactive with an antibody to protein kinase C-gamma, is rapidly modified upon cell activation: initial characterization in neutrophils and their cytoplasts.

J I Smallwood, S E Malawista

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

J I SmallwoodDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
S E Malawista
Yale University · US

Funding

BIOPHYSICAL MECHANISMS IN LEUKOCYTES AND MELANOCYTESR01AR010493 · NIAMS · YALE UNIVERSITY · PI MALAWISTA, STEPHEN E. · 1987 to 2003
$1.6M
NIAMS NIH HHS AR-10493
6 · The paper itself

Abstract

On immunoblots of human neutrophil cytoplasts (U-CYT), a previously undescribed 97 kDa protein was revealed by intense and selective reaction with an antibody that was initially raised to recognize PKC-gamma. Denoted "gamma-rp" for gamma-related protein, this acidic cytosolic protein somewhat resembled the classic forms of PKC in several biochemical respects. Appearing as a doublet on low-percentage SDS-PAGE gels, both its mobility and staining pattern were rapidly altered by treatment of U-CYT with either phorbol ester or chemotactic peptide. Whole neutrophil gamma-rp was detectable only after TCA precipitation of intact cells. It was also detectable in human platelets, lymphocytes, and neutrophil-like differentiated HL60 cells, but not in fibroblasts, erythrocytes, monocytes, or monocyte-like differentiated HL60 cells. Our data suggest that gamma-rp merits further study as a potential participant in cellular activation, and as a possible structural or functional relative of PKC.

Indexed as

Neutrophil ActivationAdolescentAnimalsAntibodiesBlood PlateletsBlood ProteinsChromatography, AgaroseChromatography, DEAE-CelluloseCytosolElectrophoresis, Polyacrylamide GelHL-60 CellsHumansImmunoblottingIsoenzymesLymphocytesNeutrophilsAntibodiesBlood ProteinsIsoenzymesProtein Kinase Cprotein kinase C gamma

Identifiers

PMID9484647
OpenAlexW265682789

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.