ArticleJournal of virology1998
Role of actin microfilaments in Black Creek Canal virus morphogenesis.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 85 citations in OpenAlex.
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- Small G Rac1 is involved in replication cycle of dengue serotype 2 virus in EAhy926 cells via the regulation of actin cytoskeleton.Science China. Life sciences · 2016Article
- Hantaviral Proteins: Structure, Functions, and Role in Hantavirus Infection.Frontiers in microbiology · 2015Review
- Antigenic properties of N protein of hantavirus.Viruses · 2014Review
- Viperin, MTAP44, and protein kinase R contribute to the interferon-induced inhibition of Bunyamwera Orthobunyavirus replication.Journal of virology · 2012Article
- Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein.Journal of virology · 2012Article
- Characterization of two substrains of Puumala virus that show phenotypes that are different from each other and from the original strain.Journal of virology · 2011Article
- Recent advances in hantavirus molecular biology and disease.Advances in applied microbiology · 2011Article
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- Roles of small GTPase Rac1 in the regulation of actin cytoskeleton during dengue virus infection.PLoS neglected tropical diseases · 2010Article
- A global perspective on hantavirus ecology, epidemiology, and disease.Clinical microbiology reviews · 2010Review
- Hantaviruses: an emerging public health threat in India? A review.Journal of biosciences · 2008Review
- NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein.The Journal of biological chemistry · 2008Article
- Dynein-dependent transport of the hantaan virus nucleocapsid protein to the endoplasmic reticulum-Golgi intermediate compartment.Journal of virology · 2007Article
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- Oligomerization of hantavirus nucleocapsid protein: analysis of the N-terminal coiled-coil domain.Journal of virology · 2006Article
- Packaging of actin into Ebola virus VLPs.Virology journal · 2005Article
- The hantavirus nucleocapsid protein recognizes specific features of the viral RNA panhandle and is altered in conformation upon RNA binding.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have investigated the involvement of cytoskeletal proteins in the morphogenesis of Black Creek Canal virus (BCCV), a New World hantavirus. Immunofluorescent staining of BCCV-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin D, an actin microfilament-depolymerizing drug. Double immunofluorescence staining of BCCV-infected Vero cells with anti-BCCV nucleocapsid (N) monoclonal antibody and phalloidin revealed a colocalization of the BCCV N protein with actin microfilaments. A similar, though less prominent, filamentous pattern was observed in BHK21 cells transiently expressing the BCCV N protein alone but not in cells expressing the BCCV G1 and G2 glycoproteins. Moreover, the association of the N protein with actin microfilaments was confirmed by coimmunoprecipitation with beta-actin-specific antibody. Treatment of the BCCV-infected Vero cells at 3 days postinfection with cytochalasin D decreased the yield of released BCCV by 94% relative to the yield from untreated cells. Pretreatment of Vero cells with cytochalasin D prior to and during BCCV adsorption and entry had no effect on the outcome of virus production. These results indicate that actin filaments may play an important role in hantavirus assembly and/or release.
Indexed as
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.