ArticleMolecular and cellular biology1998
Raf and fibroblast growth factor phosphorylate Elk1 and activate the serum response element of the immediate early gene pip92 by mitogen-activated protein kinase-independent as well as -dependent signaling pathways.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 66 citations in OpenAlex.
- Loss of ELK1 has differential effects on age-dependent organ fibrosis.The international journal of biochemistry & cell biology · 2020Article
- Prenylation differentially inhibits insulin-dependent immediate early gene mRNA expression.Biochemical and biophysical research communications · 2016Article
- The immediate early gene Ier2 promotes tumor cell motility and metastasis, and predicts poor survival of colorectal cancer patients.Oncogene · 2012Article
- Transcriptional regulation of flotillins by the extracellularly regulated kinases and retinoid X receptor complexes.PloS one · 2012Article
- FGF19 regulates cell proliferation, glucose and bile acid metabolism via FGFR4-dependent and independent pathways.PloS one · 2011Article
- Article
- CXCL5 promotes prostate cancer progression.Neoplasia (New York, N.Y.) · 2008Article
- Coxsackievirus B3 infection induces cyr61 activation via JNK to mediate cell death.Journal of virology · 2004Article
- Gene expression during the priming phase of liver regeneration after partial hepatectomy in mice.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- FGF induces a switch in death receptor pathways in neuronal cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity.Molecular and cellular biology · 2001Article
- Direct evidence that ERK regulates the production/secretion of interleukin-2 in PHA/PMA-stimulated T lymphocytes.Immunology · 1999Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Previous studies have shown that a mitogen activated protein (MAP) kinase (MEK)-independent signaling pathway is required by activated Raf or fibroblast-derived growth factor (FGF) for the differentiation of rat hippocampal neuronal H19-7 cells. We now demonstrate that both Raf and FGF similarly induce prolonged transcription and translation of the immediate early gene pip92 in the absence of activation of the MAP kinases (MAPKs) ERK1 and ERK2. To determine the mechanism by which this occurs and to identify novel Raf-activated signaling pathways, we investigated the induction of the pip92 promoter by both FGF and an estradiol-activated Raf-1-estrogen receptor fusion protein (deltaRaf-1:ER) in H19-7 cells. Deletion analysis of the pip92 promoter indicated that activation by the MAPK-independent pathway occurs primarily within the region containing a serum response element (SRE). Further analysis of the SRE by using a heterologous thymidine kinase promoter showed that both an Ets and CArG-like site are required. Elk1, which binds to the Ets site, was phosphorylated both in vitro and in vivo by the MAPK-independent pathway, and phosphorylation of an Elk1-GAL4 fusion protein by this pathway was sufficient for transactivation. Finally, at least two Elk1 kinases were fractionated by gel filtration, and analysis by an in-gel kinase assay revealed at least three novel Raf-activated Elk1 kinases. These results indicate that both FGF and Raf activate MAPK-independent kinases that can stimulate Elk1 phosphorylation and immediate early gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.