ArticleMolecular and cellular biology1998
A sequence-specific RNA-binding protein complements apobec-1 To edit apolipoprotein B mRNA.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Targeted RNA base editing for therapeutic: mechanisms and advances.Pharmaceutical science advances · 2025Review
- A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma Cells.International journal of molecular sciences · 2024Article
- Precision RNA base editing with engineered and endogenous effectors.Nature biotechnology · 2023Review
- A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits.Nature communications · 2018Article
- APOBEC1 complementation factor (A1CF) is dispensable for C-to-U RNA editing in vivo.RNA (New York, N.Y.) · 2017Article
- Identification of DNA cleavage- and recombination-specific hnRNP cofactors for activation-induced cytidine deaminase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells.RNA biology · 2015Article
- Detection of RNA-Protein Interactions Using Tethered RNA Affinity Capture.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Host restriction of murine gammaherpesvirus 68 replication by human APOBEC3 cytidine deaminases but not murine APOBEC3.Virology · 2014Article
- APOBEC1-mediated editing and attenuation of herpes simplex virus 1 DNA indicate that neurons have an antiviral role during herpes simplex encephalitis.Journal of virology · 2011Article
- Efficient detection of RNA-protein interactions using tethered RNAs.Nucleic acids research · 2011Article
- Functional characterization of APOBEC-1 complementation factor phosphorylation sites.Biochimica et biophysica acta · 2007Article
- Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factor.Nucleic acids research · 2006Article
- Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality.Molecular and cellular biology · 2005Article
- The circadian RNA-binding protein CHLAMY 1 represents a novel type heteromer of RNA recognition motif and lysine homology domain-containing subunits.Eukaryotic cell · 2004Article
- A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcripts.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Identification of domains in apobec-1 complementation factor required for RNA binding and apolipoprotein-B mRNA editing.RNA (New York, N.Y.) · 2002Article
- cis Recognition elements in plant mitochondrion RNA editing.Molecular and cellular biology · 2001Article
- Phosphorylation is a regulatory mechanism in apolipoprotein B mRNA editing.The Biochemical journal · 2001Article
- Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The editing of apolipoprotein B (apo-B) mRNA involves the site-specific deamination of cytidine to uracil. The specificity of editing is conferred by an 11-nucleotide mooring sequence located downstream from the editing site. Apobec-1, the catalytic subunit of the editing enzyme, requires additional proteins to edit apo-B mRNA in vitro, but the function of these additional factors, known as complementing activity, is not known. Using RNA affinity chromatography, we show that the complementing activity binds to a 280-nucleotide apo-B RNA in the absence of apobec-1. The activity did not bind to the antisense strand or to an RNA with three mutations in the mooring sequence. The eluate from the wild-type RNA column contained a 65-kDa protein that UV cross-linked to apo-B mRNA but not to the triple-mutant RNA. This protein was not detected in the eluates from the mutant or the antisense RNA columns. Introduction of the mooring sequence into luciferase RNA induced cross-linking of the 65-kDa protein. A 65-kDa protein that interacted with apobec-1 was also detected by far-Western analysis in the eluate from the wild-type RNA column but not from the mutant RNA column. For purification, proteins were precleared on the mutant RNA column prior to chromatography on the wild-type RNA column. Silver staining of the affinity-purified fraction detected a single prominent protein of 65 kDa. Our results suggest that the complementing activity may function as the RNA-binding subunit of the holoenzyme.
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