ArticleProceedings of the National Academy of Sciences of the United States of America1998
Unexpected frameshifts from gene to expressed protein in a phage-displayed peptide library.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Review
- Review
- Characterization of a virulence-modifying protein ofFrontiers in microbiology · 2022Article
- Phage Display Derived Monoclonal Antibodies: From Bench to Bedside.Frontiers in immunology · 2020Review
- Mining gut microbiome oligopeptides by functional metaproteome display.Scientific reports · 2016Article
- Isolation and characterization of antibody fragments selective for toxic oligomeric tau.Neurobiology of aging · 2015Article
- Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display.BMC biotechnology · 2012Article
- Design and screening of M13 phage display cDNA libraries.Molecules (Basel, Switzerland) · 2011Review
- Article
- Frameshifting in the p6 cDNA phage display system.Molecules (Basel, Switzerland) · 2010Article
- Characterizing monoclonal antibody epitopes by filtered gene fragment phage display.The Biochemical journal · 2005Article
- A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without recombinant protein.Nucleic acids research · 2003Article
- Selecting open reading frames from DNA.Genome research · 2003Article
- Peptides identify multiple hotspots within the ligand binding domain of the TNF receptor 2.Proteome science · 2003Article
- Mimotopes and proteome analyses using human genomic and cDNA epitope phage display.Comparative and functional genomics · 2002Article
- Genetic analysis of the basis of translation in the -1 frame of an unusual non-ORF sequence isolated from phage display.Gene expression · 2002Article
- A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus.Journal of virological methods · 2001Article
- A surrogate-based approach for post-genomic partner identification.BMC biotechnology · 2001Article
- Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins.Biological procedures onlineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A library of long peptides displayed on the pIII protein of filamentous phage was used in biopanning experiments against several protein targets. We find that a large percentage of phage clones that bind specifically to a target contain peptide-encoding genes that do not have an ORF. Instead, the reading frame is either interrupted by one or more nonsuppressed stop codons, or a post-transcriptional frameshift is needed to account for the expression of the minor phage coat protein pIII. The percentage of frameshifted clones varies depending on the target. It can be as high as 90% for clones specific for soluble forms of certain cytokine receptors. Conversely, biopanning against four mAbs did not yield any frameshifted clones. Our studies focused on one clone that binds specifically to rat growth hormone binding protein (GHBP) yet does not have an ORF. A secondary peptide library containing random mutations of this sequence was constructed and panned against GHBP to optimize and correct the reading frame. In the last round (round two) of panning with this library, none of the phage clones that bound to GHBP had an ORF. However, careful analysis of these clones allowed us to design a synthetic peptide capable of binding to GHBP. The results of this study indicate that ORFs are not required to obtain gene expression of the minor coat protein of filamentous phage and suggest that some ORF- clones may have a selective advantage over the clones having ORFs.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.