Evidence map›Paper›PMID 19937758›Full record

ArticleBiopolymers2010

Impact of alpha-hydroxy-propanodeoxyguanine adducts on DNA duplex energetics: opposite base modulation and implications for mutagenicity and genotoxicity.

Conceição A S A Minetti, David P Remeta, Francis Johnson, Charles R Iden, Kenneth J Breslauer

Open access · greenAbstract read
In one paragraph

Article in Biopolymers, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Review
  3. Origin and Fate of Acrolein in Foods.Foods (Basel, Switzerland) · 2022
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Conceição A S A MinettiDepartment of Chemistry and Chemical Biology, Rutgers-The State University of New Jersey, Piscataway, NJ 08854, USA.
David P Remeta
Francis Johnson
Charles R Iden
Kenneth J Breslauer
Rutgers, The State University of New Jersey · USStony Brook University · USRutgers Cancer Institute of New Jersey

Funding

THERMODYNAMIC PROPERTIES OF EXOCYCLIC DNA ADDUCTSP01CA047995 · NCI · STATE UNIVERSITY NEW YORK STONY BROOK · PI GROLLMAN, ARTHUR PATRICK · 1990 to 2009
$9.8M
DNA STABILITY AND FLEXIBILITY: A THERMODYNAMIC STUDYR01GM023509 · NIGMS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI BRESLAUER, KENNETH J. · 1985 to 1998
–
DRUG-DNA INTERACTIONS: THE THERMODYNAMICS OF RECOGNITIONR01GM034469 · NIGMS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI BRESLAUER, KENNETH J. · 1985 to 1994
–
NCI NIH HHS CA47995NIGMS NIH HHS GM23509NIGMS NIH HHS GM34469NIGMS NIH HHS R01 GM023509
6 · The paper itself

Abstract

Acrolein is an alpha,beta-unsaturated aldehyde that is a major environmental pollutant, as well as a product of cellular metabolism. DNA bases react with acrolein to form two regioisomeric exocyclic guanine adducts, namely gamma-hydroxy-propanodeoxyguanosine (gamma-OH-PdG) and its positional isomer alpha-hydroxy-propanodeoxyguanosine (alpha-OH-PdG). The gamma-OH-PdG isomer adopts a ring-opened conformation with minimal structural perturbation of the DNA host duplex. Conversely, the alpha-OH-PdG isomer assumes a ring-closed conformation that significantly disrupts Watson-Crick base-pair alignments within the immediate vicinity of the damaged site. We have employed a combination of calorimetric and spectroscopic techniques to characterize the thermodynamic origins of these lesion-induced structural alterations. Specifically, we have assessed the energetic impact of alpha-OH-PdG centered within an 11-mer duplex by hybridizing the adduct-containing oligonucleotide with its complementary strand harboring a central base N [where N = C or A], yielding a pair of duplexes containing the nascent lesion (alpha-OH-PdG.C) or mismatched adduct (alpha-OH-PdG.A), respectively. Our data reveal that the nascent lesion is highly destabilizing, whereas its mismatched counterpart partially ameliorates alpha-OH-PdG-induced destabilization. Collectively, our data provide energetic characterizations of the driving forces that modulate error-free versus error-prone DNA translesion synthesis. The biological implications of our findings are discussed in terms of energetically probing acrolein-mediated mutagenicity versus adduct-induced genotoxicity.

Indexed as

DNA DamageMutagenesisAcroleinCalorimetry, Differential ScanningCircular DichroismDNADNA AdductsGuanineOligonucleotidesThermodynamicsAcroleinDNADNA AdductsGuanineOligonucleotides

Identifiers

PMID19937758
PMCPMC2891022
OpenAlexW2166254930

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.